Witryna3 lut 2024 · The MACSima Imaging System is a fully automated instrument combining liquid handling with widefield microscopy for cyclic immunofluorescence imaging. In brief, staining cycles consisted of the following automated steps: immunofluorescent staining, sample washing, multi-field imaging, and signal erasure (photobleaching or … Witryna19 maj 2024 · Highly multiplex fluorescence based systems, such as, the MACSima Imaging Cyclic Staining (MICS) can detect hundreds of fluorescently-tagged antibodies at a sub-cellular level in a single tissue section through the use of automated cycles of sequential staining, image acquisition, and signal erasure through photobleaching …
The workflow of cyclic multiplexed-immunofluorescence (cmIF). (a ...
WitrynaThe MACSima™ Instrument is a fully automated imaging system based on fluorescence microscopy. It's MICS (MACSima™ Imaging Cyclic Staining (MICS) technology … Witryna24 lis 2024 · Spatially resolved proteomics requires a highly multiplexed imaging modality. Cyclic imaging techniques, which repeat staining, imaging, and signal … bing girl on search bar
Cyclic imaging with the MACSima Imaging Platform. (a) …
Witryna27 cze 2024 · The process can be divided into three stages: (1) tissue staining with tyramide-barcodes and barcode-conjugated antibodies, (2) cyclical fluorescence imaging, and (3) image processing and ... Witryna13 mar 2024 · Light microscopy and fluorescent microscopy make it possible to detect only a handful of targets simultaneously because of spectral overlap. To increase the number of targets imaged, cyclic microscopy methods perform a variation of a cyclic process that involves several steps: (1) tissue staining using tagged antibodies, (2) … WitrynaA cyclic staining, imaging, fluorophore inactivation protocol for multiplexed protein imaging was employed as shown previously 33. A panel of 33 antibodies was used … bing gift cards