WebFeb 8, 2024 · Compared to the genome of the related phage, Hafnia phage Ca genome contains extra genes including DNA mimic ocr, dGTP triphosphohydrolase inhibitor, … WebIn 2001, Kicska et al. developed immucillin H, now better known as forodesine, as a small molecule PNP inhibitor (PNP-I). 4 The intention of the authors was to kill lymphocytes by elevating intracellular dG and consequently dGTP levels and to thereby eliminate leukemic cells. Forodesine was subsequently tested in clinical trials and was found ...
The crystal structure of dGTPase reveals the molecular basis of dGTP ...
WebDeoxyguanosinetriphosphate triphosphohydrolase (dGTPase) is a triphosphohydrolase that has a preference for dGTP over other dNTPs, they hydrolase dGTP to form … WebDeoxyguanosinetriphosphate triphosphohydrolase (dGTPase) is a triphosphohydrolase that has a preference for dGTP over other dNTPs, they hydrolase dGTP to form deoxyguanosine and triphosphate. Escherichia coli dGTPase (Dgt) has been shown to have a strong single-stranded DNA-binding (ssDNA) activity [PMID:25694425] which is … canfield sign in
RCSB PDB - 7U66: Structure of E. coli dGTPase bound to T7 …
WebNucleotide hydrolases are known to hydrolyze not only noncanonical dNTPs to reduce the risk of mutation, but also canonical dNTPs to maintain the dNTP concentrations in the cell. dGTP triphosphohydrolase from Escherichia coli is known as an enzyme that hydrolyzes dGTP. Recently, we identified a triphosphohydrolase from Thermus thermophilus HB8 … The dNTPase activity of SAMHD1 is stimulated by dGTP24,25. dGTP, but not the other dNTPs, is also required for the formation/maintenance of the SAMHD1 tetramer (Fig. 1). A detailed view of the dGTP-binding site provides an explanation for the stabilization of the SAMHD1c′ tetramer by dGTP. Two dGTP … See more The SAM domain and its amino-terminal region are not required for tetramer formation or the dNTPase activity of SAMHD1 (refs 27, 28). However, SAMHD1c (120–626) … See more We have co-crystallized SAMHD1c′ with its dGTP/dATP substrates and solved the structure by the molecular replacement method, using 3U1N … See more To determine the importance for tetramer formation of dGTP/dATP binding in the allosteric site, we tested several SAMHD1 mutants. These … See more The substrate-binding pocket in the active site is formed by amino acids in the major lobe (Fig. 5b). Extensive interactions between the substrate dGTP and surrounding amino … See more WebApr 1, 1990 · The dgt gene lies immediately upstream of htrA and 6 kilobases from dapD, in the same region as the optA1 mutation. The dgt structural gene is 1515 base pairs, … canfield single towel bar